Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis.

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Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis. / Petersson, Linn; Dexlin-Mellby, Linda; Bengtsson, Anders; Sturfelt, Gunnar; Borrebaeck, Carl A K; Wingren, Christer.

In: Lab on a Chip, Vol. 14, No. 11, 2014, p. 1931-1942.

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Petersson, Linn ; Dexlin-Mellby, Linda ; Bengtsson, Anders ; Sturfelt, Gunnar ; Borrebaeck, Carl A K ; Wingren, Christer. / Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis. In: Lab on a Chip. 2014 ; Vol. 14, No. 11. pp. 1931-1942.

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TY - JOUR

T1 - Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis.

AU - Petersson, Linn

AU - Dexlin-Mellby, Linda

AU - Bengtsson, Anders

AU - Sturfelt, Gunnar

AU - Borrebaeck, Carl A K

AU - Wingren, Christer

PY - 2014

Y1 - 2014

N2 - In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first clinical application targeting SLE nephritis, a severe chronic autoimmune connective tissue disorder, as the model disease. To this end, crude, directly biotinylated serum samples were targeted. The results showed that the miniaturized and multiplexed array platform displayed adequate performance, and that SLE-associated serum biomarker panels reflecting the disease process could be deciphered, outlining the use of miniaturized antibody arrays for disease proteomics and biomarker discovery.

AB - In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first clinical application targeting SLE nephritis, a severe chronic autoimmune connective tissue disorder, as the model disease. To this end, crude, directly biotinylated serum samples were targeted. The results showed that the miniaturized and multiplexed array platform displayed adequate performance, and that SLE-associated serum biomarker panels reflecting the disease process could be deciphered, outlining the use of miniaturized antibody arrays for disease proteomics and biomarker discovery.

U2 - 10.1039/c3lc51420j

DO - 10.1039/c3lc51420j

M3 - Article

VL - 14

SP - 1931

EP - 1942

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

T2 - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0189

IS - 11

ER -