Mutated cylindromatosis gene affects the functional state of dendritic cells

Research output: Contribution to journalArticle

Abstract

Cylindromatosis gene (CYLD) is a ubiquitously expressed deubiquitinating enzyme, which interacts with members of the NF-kappa B signaling pathway and attenuates NF-kappa B and JNK signaling. Here, we report that DC derived from transgenic mice, which solely express a naturally occurring CYLD isoform (CYLDex7/8), display a higher content of nuclear RelB and express elevated levels of NF-kappa B family members as well as of known NF-kappa B-target genes comprising costimulatory molecules and pro-inflammatory cytokines, as compared with WT DC. Accordingly, unstimulated CYLDex7/8 DC exhibited a significantly higher primary allogenic T-cell stimulatory capacity than WT DC and exerted no tolerogenic activity. Transduction of unstimulated CYLDex7/8 DC with relB-specific shRNA reduced their T-cell stimulatory capacity. Treatment with the synthetic glucocorticoid dexamethasone known to inhibit NF-kappa B and AP-1 activity reverted the pro-immunogenic phenotype and function of CYLDex7/8 DC and re-established their tolerogenic function. DC derived from CYLD knockout mice showed no functional alterations compared with WT DC. Therefore, although complete loss of CYLD may be compensated for by other endogenous NF-kappa B inhibitors, CYLDex7/8 acts in a dominant negative manner. Our findings raise the question of whether genetic defects associated with increased NF-kappa B activity may result in disturbed maintenance of peripheral tolerance.

Details

Authors
  • Matthias Bros
  • Nadine Dexheimer
  • Verena Besche
  • Joumana Masri
  • Stefanie Trojandt
  • Nadine Hoevelmeyer
  • Sonja Reissig
  • Ramin Massoumi
  • Stephan Grabbe
  • Ari Waisman
  • Angelika B. Reske-Kunz
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • Transgenic/knockout mice, Tolerance, DC, Autoimmunity, Costimulation
Original languageEnglish
Pages (from-to)2848-2857
JournalEuropean Journal of Immunology
Volume40
Issue number10
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes