NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg–protoporphyrin monomethyl ester cyclase

Research output: Contribution to journalArticle

Abstract

Abstract The chloroplast-localized NADPH-dependent thioredoxin
reductase (NTRC) has been found to be able to reduce
hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show
that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis
and accumulate intermediates preceding protochlorophyllide
formation. A specific involvement of NTRC during
biosynthesis of protochlorophyllide is indicated from in vitro aerobic
cyclase assays in which the conversion of Mg–protoporhyrin
monomethyl ester into protochlorophyllide is stimulated by addition
of the NTRC/2-Cys peroxiredoxin system. These findings
support the hypothesis that this NADPH-dependent hydrogen
peroxide scavenging system is particularly important during
periods with limited reducing power from photosynthesis, e.g. under
chloroplast biogenesis.

Details

Authors
  • Anne Stenbaek
  • Andreas Hansson
  • Ragna Peterson Wulff
  • Mats Hansson
  • Karl-Josef Dietz
  • Poul Erik Jensen
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Gene co-expression, Chloroplast biogenesis, Biosynthetic pathway, Hydrogen peroxide scavenging, Arabidopsis thaliana, Aerobic cyclase
Original languageEnglish
Pages (from-to)2773-2778
JournalFEBS Letters
Volume582
Issue number18
Publication statusPublished - 2008
Publication categoryResearch
Peer-reviewedYes