Naturally processed T cell epitopes from human glutamic acid decarboxylase identified using mice transgenic for the type 1 diabetes- associated human MHC class II allele, DRB1*0401

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Bibtex

@article{c1f7e3689df046de9a288acd548b9440,
title = "Naturally processed T cell epitopes from human glutamic acid decarboxylase identified using mice transgenic for the type 1 diabetes- associated human MHC class II allele, DRB1*0401",
abstract = "The identification of class II binding peptide epitopes from autoimmune disease-related antigens is an essential step in the development of antigen- specific immune modulation therapy. In the case of type 1 diabetes, T cell and B cell reactivity to the autoantigen glutamic acid decarboxylase 65 (GAD65) is associated with disease development in humans and in nonobese diabetic (NOD) mice. In this study, we identify two DRB1*0401-restricted T cell epitopes from human GAD65, 274-286, and 115-127. Both peptides are immunogenic in transgenic mice expressing functional DRB1*0401 MHC class II molecules but not in nontransgenic littermates. Processing of GAD65 by antigen presenting cells (APC) resulted in the formation of DRB1*0401 complexes loaded with either the 274-286 or 115-127 epitopes, suggesting that these naturally derived epitopes may be displayed on APC recruited into pancreatic islets. The presentation of these two T cell epitopes in the islets of DRB1*0401 individuals who are at risk for type 1 diabetes may allow for antigen-specific recruitment of regulatory cells to the islets following peptide immunization.",
keywords = "autoimmunity, diabetes mellitus, insulin-dependent, MHC class II, mice, inbred NOD, peptide autoantigens",
author = "Wicker, {Linda S.} and Chen, {Shiow Ling} and Nepom, {Gerald T.} and Elliott, {John F.} and Freed, {Daniel C.} and Alka Bansal and Song Zheng and Andrew Herman and {\AA}ke Lernmark and Zaller, {Dennis M.} and Peterson, {Laurence B.} and Rothbard, {Jonathan B.} and Richard Cummings and Whiteley, {Phyllis J.}",
year = "1996",
month = dec,
day = "1",
doi = "10.1172/JCI119079",
language = "English",
volume = "98",
pages = "2597--2603",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "Am Soc Clin Investig",
number = "11",

}