Neuroprotective effects of topical CB1 agonist WIN 55212-2 on retinal ganglion cells after acute rise in intraocular pressure induced ischemia in rat

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Bibtex

@article{6ccbf38416d84b5887bf2bbad3f94734,
title = "Neuroprotective effects of topical CB1 agonist WIN 55212-2 on retinal ganglion cells after acute rise in intraocular pressure induced ischemia in rat",
abstract = "Neuroprotection in retinal experimental work consists primarily of preventing retinal ganglion cell (RGC) loss after exposure to a hostile event. We have studied the neuroprotective effect on RGCs in an ischemia-reperfusion model by activation of the cannabinoid receptor CB1 using topical application of WIN 55212-2. Intraocular pressure (IOP) was increased by continuous infusion of phosphate buffer saline (PBS) into the anterior chamber of the eye. Mean intraocular pressure was increased up to 88.5 ± 0.29 mm Hg (control normal IOP 15.1 ± 0.25 mm Hg), for 35 min. Animals were distributed in 3 groups. Left eyes underwent acute rise in intraocular pressure. First group was treated with topical Tocrisolve™ 100 in both eyes. Second group was treated with 1{\%} solution of CB1 agonist WIN 55212-2 in both eyes. Third group was treated with WIN 55212-2 1{\%} and CB1 antagonist AM 251 1{\%} solutions in both eyes. Subsequently, RGCs were immunolabeled with Brn3a and automated quantification of retinal mosaics of RGCs were performed. The ischemic damage led to a mean loss in RGC density of 12.33{\%}. After topic administration of WIN 55212-2, mean loss of RGCs was of 2.45{\%}. Co-treatment with CB1 antagonist AM 251 abolished almost completely the neuroprotective effect of WIN 55212-2. Topic 1{\%} WIN 55212-2 showed a neuroprotective effect on RGC degeneration after ischemia-reperfusion without pre-activation of CB1 receptors.",
keywords = "Administration, Topical, Animals, Benzoxazines, Cell Count, Disease Models, Animal, Female, Intraocular Pressure, Morpholines, Naphthalenes, Neuroprotective Agents, Ocular Hypertension, Piperidines, Pyrazoles, Rats, Rats, Sprague-Dawley, Receptor, Cannabinoid, CB1, Reperfusion Injury, Retinal Diseases, Retinal Ganglion Cells, Tonometry, Ocular, Journal Article, Research Support, Non-U.S. Gov't",
author = "Sergio Pinar-Sueiro and {Zorrilla Hurtado}, {Jos{\'e} {\'A}ngel} and Patricia Veiga-Crespo and Sharma, {Sansar C} and Elena Vecino",
note = "Copyright {\circledC} 2013 Elsevier Ltd. All rights reserved.",
year = "2013",
month = "5",
doi = "10.1016/j.exer.2013.02.009",
language = "English",
volume = "110",
pages = "55--8",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Elsevier",

}