New directions of miniaturization within the proteomics research area

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New directions of miniaturization within the proteomics research area. / Marko-Varga, György; Nilsson, Johan; Laurell, Thomas.

In: Electrophoresis, Vol. 24, No. 21, 2003, p. 3521-3532.

Research output: Contribution to journalReview article

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TY - JOUR

T1 - New directions of miniaturization within the proteomics research area

AU - Marko-Varga, György

AU - Nilsson, Johan

AU - Laurell, Thomas

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)

PY - 2003

Y1 - 2003

N2 - An overview of the current trends within protein expression profiling is given where multidimensional separation of both gel- and liquid-phase techniques linked to mass spectrometry is viewed as a major route in the global proteome mapping. A clear trend in these biochemical developments is the effort to sequence and identify low-abundant protein expressions where assay miniaturization and integrated sample processing plays a central role. Two areas of miniaturization within the proteomics field are addressed: (i) sample cleanup and enrichment, and (ii) silicon microstructure developments for protein chip microarrays.

AB - An overview of the current trends within protein expression profiling is given where multidimensional separation of both gel- and liquid-phase techniques linked to mass spectrometry is viewed as a major route in the global proteome mapping. A clear trend in these biochemical developments is the effort to sequence and identify low-abundant protein expressions where assay miniaturization and integrated sample processing plays a central role. Two areas of miniaturization within the proteomics field are addressed: (i) sample cleanup and enrichment, and (ii) silicon microstructure developments for protein chip microarrays.

KW - proteomics

KW - miniaturization

KW - protein expression profiling

KW - review

U2 - 10.1002/elps.200305666

DO - 10.1002/elps.200305666

M3 - Review article

VL - 24

SP - 3521

EP - 3532

JO - Electrophoresis

T2 - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 21

ER -