Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins

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Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins. / Landrein, Benoit; Formosa-Jordan, Pau; Malivert, Alice; Schuster, Christoph; Melnyk, Charles W.; Yang, Weibing; Turnbull, Colin; Meyerowitz, Elliot M.; Locke, James C.W.; Jönsson, Henrik.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 6, 06.02.2018, p. 1382-1387.

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Landrein, B, Formosa-Jordan, P, Malivert, A, Schuster, C, Melnyk, CW, Yang, W, Turnbull, C, Meyerowitz, EM, Locke, JCW & Jönsson, H 2018, 'Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 6, pp. 1382-1387. https://doi.org/10.1073/pnas.1718670115

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Landrein, Benoit ; Formosa-Jordan, Pau ; Malivert, Alice ; Schuster, Christoph ; Melnyk, Charles W. ; Yang, Weibing ; Turnbull, Colin ; Meyerowitz, Elliot M. ; Locke, James C.W. ; Jönsson, Henrik. / Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 6. pp. 1382-1387.

RIS

TY - JOUR

T1 - Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins

AU - Landrein, Benoit

AU - Formosa-Jordan, Pau

AU - Malivert, Alice

AU - Schuster, Christoph

AU - Melnyk, Charles W.

AU - Yang, Weibing

AU - Turnbull, Colin

AU - Meyerowitz, Elliot M.

AU - Locke, James C.W.

AU - Jönsson, Henrik

PY - 2018/2/6

Y1 - 2018/2/6

N2 - The shoot apical meristem (SAM) is responsible for the generation of all the aerial parts of plants. Given its critical role, dynamical changes in SAM activity should play a central role in the adaptation of plant architecture to the environment. Using quantitative microscopy, grafting experiments, and genetic perturbations, we connect the plant environment to the SAM by describing the molecular mechanism by which cytokinins signal the level of nutrient availability to the SAM. We show that a systemic signal of cytokinin precursors mediates the adaptation of SAM size and organogenesis rate to the availability of mineral nutrients by modulating the expression of WUSCHEL, a key regulator of stem cell homeostasis. In time-lapse experiments, we further show that this mechanism allows meristems to adapt to rapid changes in nitrate concentration, and thereby modulate their rate of organ production to the availability of mineral nutrients within a few days. Our work sheds light on the role of the stem cell regulatory network by showing that it not only maintains meristem homeostasis but also allows plants to adapt to rapid changes in the environment.

AB - The shoot apical meristem (SAM) is responsible for the generation of all the aerial parts of plants. Given its critical role, dynamical changes in SAM activity should play a central role in the adaptation of plant architecture to the environment. Using quantitative microscopy, grafting experiments, and genetic perturbations, we connect the plant environment to the SAM by describing the molecular mechanism by which cytokinins signal the level of nutrient availability to the SAM. We show that a systemic signal of cytokinin precursors mediates the adaptation of SAM size and organogenesis rate to the availability of mineral nutrients by modulating the expression of WUSCHEL, a key regulator of stem cell homeostasis. In time-lapse experiments, we further show that this mechanism allows meristems to adapt to rapid changes in nitrate concentration, and thereby modulate their rate of organ production to the availability of mineral nutrients within a few days. Our work sheds light on the role of the stem cell regulatory network by showing that it not only maintains meristem homeostasis but also allows plants to adapt to rapid changes in the environment.

KW - Arabidopsis

KW - Cytokinin hormones

KW - Plant development

KW - Plant nutrition

KW - Shoot apical meristem

U2 - 10.1073/pnas.1718670115

DO - 10.1073/pnas.1718670115

M3 - Article

VL - 115

SP - 1382

EP - 1387

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

SN - 1091-6490

IS - 6

ER -