NMR quantification of diffusional exchange in cell suspensions with relaxation rate differences between intra and extracellular compartments

Research output: Contribution to journalArticle

Abstract

Water transport across cell membranes can be measured non-invasively with diffusion NMR. We present a method to quantify the intracellular lifetime of water in cell suspensions with short transverse relaxation times, T2, and also circumvent the confounding effect of different T2 values in the intra- and extracellular compartments. Filter exchange spectroscopy (FEXSY) is specifically sensitive to exchange between compartments with different apparent diffusivities. Our investigation shows that FEXSY could yield significantly biased results if differences in T2 are not accounted for. To mitigate this problem, we propose combining FEXSY with diffusion-relaxation correlation experiment, which can quantify differences in T2 values in compartments with different diffusivities. Our analysis uses a joint constrained fitting of the two datasets and considers the effects of diffusion, relaxation and exchange in both experiments. The method is demonstrated on yeast cells with and without human aquaporins.

Details

Authors
Organisations
External organisations
  • CR Development AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Other Basic Medicine
Original languageEnglish
Article numbere0177273
JournalPLoS ONE
Volume12
Issue number5
Publication statusPublished - 2017 May 1
Publication categoryResearch
Peer-reviewedYes