Nonrandom chromosomal aberrations and cytogenetic heterogeneity in gallbladder carcinomas

Research output: Contribution to journalArticle

Abstract

Chromosome banding analysis of 11 short-term cultured gallbladder carcinomas revealed acquired clonal aberrations in seven tumors (five primary and two metastases). Three of these had one clone, whereas the remaining four were cytogenetically heterogeneous, displaying two to seven aberrant clones. Of a total of 21 abnormal clones, 18 had highly complex karyotypes and three exhibited simple numerical deviations. Double minutes and homogeneously staining regions were observed in one and two carcinomas, respectively. To characterize the karyotypic profile of gallbladder cancer more precisely, we have combined the present findings with our three previously reported cases, thereby providing the largest cytogenetic database on this tumor type to date. A total of 287 chromosomal breakpoints were identified, 251 of which were found in the present study. Chromosome 7 was rearranged most frequently, followed by chromosomes 1, 3, 11, 6, 5, and 8. The bands preferentially involved were 1p32, 1p36, 1q32, 3p21, 6p21, 7p13, 7q11, 7q32, 19p13, 19q13, and 22q13. Nine recurrent abnormalities could, for the first time, be identified in gallbladder carcinoma: del(3)(p13), i(5)(p10), del(6)(q13), del(9)(p13), del(16)(q22), del(17)(p11), i(17)(q10), del(19)(p13), and i(21)(q10). The most common partial or whole-arm gains involved 3q, 5p, 7p, 7q, 8q, 11q, 13q, and 17q, and the most frequent partial or whole-arm losses affected 3p, 4q, 5q, 9p, 10p, 10q, 11p, 14p, 14q, 15p, 17p, 19p, 21p, 21q, and Xp. These chromosomal aberrations and imbalances provide some starting points for molecular analyses of genomic regions that may harbor genes of pathogenetic importance in gallbladder carcinogenesis. Genes Chromosomes Cancer 26:312-321, 1999.

Details

Authors
  • Ludmila Gorunova
  • Luis Antonio Parada
  • J. Limon
  • Yuesheng Jin
  • Magnus Hallén
  • Inga Hagerstrand
  • M. Iliszko
  • Z. Wajda
  • Bertil Johansson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical and Health Sciences

Keywords

  • Clone Cells, Middle Aged, Chromosome Aberrations, Aged, Carcinoma/ genetics, Humans, Karyotyping, Male, Genetic Heterogeneity, Female, Gallbladder Neoplasms/ genetics
Original languageEnglish
Pages (from-to)312-21
JournalGenes, Chromosomes and Cancer
Volume26
Issue number4
Publication statusPublished - 1999
Publication categoryResearch
Peer-reviewedYes