Non-specific effects of leukotriene synthesis inhibitors on HeLa cell physiology

Research output: Contribution to journalArticle

Abstract

We examined the effects of various leukotriene synthesis inhibitors on calcium signalling in HeLa cells, before and after transfection with BLT1. All of the inhibitors studied were found to reduce increases in intracellular calcium concentration induced by BLT1, but also by an ionophore or activation of various G-protein coupled receptors, regardless of BLT1 expression. In order to explore the mechanism of these apparently general effects we examined HeLa cell expression of leukotriene receptors and biosynthetic enzymes and found that the genes for key leukotriene synthesis enzymes and all of the leukotriene receptors were not expressed. Leukotrienes are involved in the pathology of a variety of cancers, and for HeLa cells leukotrienes have been reported to be important for aspects of the carcinogenic phenotype. We find that leukotriene synthesis inhibitors have non-specific effects, so careful controls are necessary to avoid interpreting non-specific effects as evidence for leukotriene involvement.

Details

Authors
  • A Sabirsh
  • Jesper Bristulf
  • Ulf Karlsson
  • Christer Owman
  • JZ Haeggstrom
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences
Original languageEnglish
Pages (from-to)431-440
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume73
Issue number6
Publication statusPublished - 2005
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Molecular Neurobiology (0131000140), Division of Infection Medicine (SUS) (013008000), Drug Target Discovery (013212045), LU Innovation System (018009040)