Novel centromeric loci of the wine and beer yeast dekkera bruxellensis CEN1 and CEN2

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Novel centromeric loci of the wine and beer yeast dekkera bruxellensis CEN1 and CEN2. / Ishchuk, Olena P.; Zeljko, Tanja Vojvoda; Schifferdecker, Anna J.; Wisén, Sofia Mebrahtu; Hagström, Åsa K.; Rozpedowska, Elzbieta; Andersen, Mikael Rørdam; Hellborg, Linda; Ling, Zhihao; Sibirny, Andrei A.; Piskur, Jure.

In: PLoS ONE, Vol. 11, No. 8, e0161741, 01.08.2016.

Research output: Contribution to journalArticle

Harvard

Ishchuk, OP, Zeljko, TV, Schifferdecker, AJ, Wisén, SM, Hagström, ÅK, Rozpedowska, E, Andersen, MR, Hellborg, L, Ling, Z, Sibirny, AA & Piskur, J 2016, 'Novel centromeric loci of the wine and beer yeast dekkera bruxellensis CEN1 and CEN2', PLoS ONE, vol. 11, no. 8, e0161741. https://doi.org/10.1371/journal.pone.0161741

APA

Ishchuk, O. P., Zeljko, T. V., Schifferdecker, A. J., Wisén, S. M., Hagström, Å. K., Rozpedowska, E., Andersen, M. R., Hellborg, L., Ling, Z., Sibirny, A. A., & Piskur, J. (2016). Novel centromeric loci of the wine and beer yeast dekkera bruxellensis CEN1 and CEN2. PLoS ONE, 11(8), [e0161741]. https://doi.org/10.1371/journal.pone.0161741

CBE

Ishchuk OP, Zeljko TV, Schifferdecker AJ, Wisén SM, Hagström ÅK, Rozpedowska E, Andersen MR, Hellborg L, Ling Z, Sibirny AA, Piskur J. 2016. Novel centromeric loci of the wine and beer yeast dekkera bruxellensis CEN1 and CEN2. PLoS ONE. 11(8):Article e0161741. https://doi.org/10.1371/journal.pone.0161741

MLA

Vancouver

Ishchuk OP, Zeljko TV, Schifferdecker AJ, Wisén SM, Hagström ÅK, Rozpedowska E et al. Novel centromeric loci of the wine and beer yeast dekkera bruxellensis CEN1 and CEN2. PLoS ONE. 2016 Aug 1;11(8). e0161741. https://doi.org/10.1371/journal.pone.0161741

Author

Ishchuk, Olena P. ; Zeljko, Tanja Vojvoda ; Schifferdecker, Anna J. ; Wisén, Sofia Mebrahtu ; Hagström, Åsa K. ; Rozpedowska, Elzbieta ; Andersen, Mikael Rørdam ; Hellborg, Linda ; Ling, Zhihao ; Sibirny, Andrei A. ; Piskur, Jure. / Novel centromeric loci of the wine and beer yeast dekkera bruxellensis CEN1 and CEN2. In: PLoS ONE. 2016 ; Vol. 11, No. 8.

RIS

TY - JOUR

T1 - Novel centromeric loci of the wine and beer yeast dekkera bruxellensis CEN1 and CEN2

AU - Ishchuk, Olena P.

AU - Zeljko, Tanja Vojvoda

AU - Schifferdecker, Anna J.

AU - Wisén, Sofia Mebrahtu

AU - Hagström, Åsa K.

AU - Rozpedowska, Elzbieta

AU - Andersen, Mikael Rørdam

AU - Hellborg, Linda

AU - Ling, Zhihao

AU - Sibirny, Andrei A.

AU - Piskur, Jure

PY - 2016/8/1

Y1 - 2016/8/1

N2 - The wine and beer yeast Dekkera bruxellensis thrives in environments that are harsh and limiting, especially in concentrations with low oxygen and high ethanol. Its different strains' chromosomes greatly vary in number (karyotype). This study isolates two novel centromeric loci (CEN1 and CEN2), which support both the yeast's autonomous replication and the stable maintenance of plasmids. In the sequenced genome of the D. bruxellensis strain CBS 2499, CEN1 and CEN2 are each present in one copy. They differ from the known "point" CEN elements, and their biological activity is retained within ~900-1300 bp DNA segments. CEN1 and CEN2 have features of both "point" and "regional" centromeres: They contain conserved DNA elements, ARSs, short repeats, one tRNA gene, and transposon-like elements within less than 1 kb. Our discovery of a miniature inverted-repeat transposable element (MITE) next to CEN2 is the first report of such transposons in yeast. The transformants carrying circular plasmids with cloned CEN1 and CEN2 undergo a phenotypic switch: They form fluffy colonies and produce three times more biofilm. The introduction of extra copies of CEN1 and CEN2 promotes both genome rearrangements and ploidy shifts, with these effects mediated by homologous recombination (between circular plasmid and genome centromere copy) or by chromosome breakage when integrated. Also, the proximity of the MITE-like transposon to CEN2 could translocate CEN2 within the genome or cause chromosomal breaks, so promoting genome dynamics. With extra copies of CEN1 and CEN2, the yeast's enhanced capacities to rearrange its genome and to change its gene expression could increase its abilities for exploiting new and demanding niches.

AB - The wine and beer yeast Dekkera bruxellensis thrives in environments that are harsh and limiting, especially in concentrations with low oxygen and high ethanol. Its different strains' chromosomes greatly vary in number (karyotype). This study isolates two novel centromeric loci (CEN1 and CEN2), which support both the yeast's autonomous replication and the stable maintenance of plasmids. In the sequenced genome of the D. bruxellensis strain CBS 2499, CEN1 and CEN2 are each present in one copy. They differ from the known "point" CEN elements, and their biological activity is retained within ~900-1300 bp DNA segments. CEN1 and CEN2 have features of both "point" and "regional" centromeres: They contain conserved DNA elements, ARSs, short repeats, one tRNA gene, and transposon-like elements within less than 1 kb. Our discovery of a miniature inverted-repeat transposable element (MITE) next to CEN2 is the first report of such transposons in yeast. The transformants carrying circular plasmids with cloned CEN1 and CEN2 undergo a phenotypic switch: They form fluffy colonies and produce three times more biofilm. The introduction of extra copies of CEN1 and CEN2 promotes both genome rearrangements and ploidy shifts, with these effects mediated by homologous recombination (between circular plasmid and genome centromere copy) or by chromosome breakage when integrated. Also, the proximity of the MITE-like transposon to CEN2 could translocate CEN2 within the genome or cause chromosomal breaks, so promoting genome dynamics. With extra copies of CEN1 and CEN2, the yeast's enhanced capacities to rearrange its genome and to change its gene expression could increase its abilities for exploiting new and demanding niches.

UR - http://www.scopus.com/inward/record.url?scp=84991508707&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0161741

DO - 10.1371/journal.pone.0161741

M3 - Article

AN - SCOPUS:84991508707

VL - 11

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 8

M1 - e0161741

ER -