Novel disease-specific promoters for use in gene therapy for Parkinson's disease.

Research output: Contribution to journalArticle

Abstract

Gene therapy is a promising therapeutic tool for Parkinson's disease (PD), but there is a lack of evaluated cell specific promoters that are relevant for the disease. We have chosen PD relevant promoter candidates for gene therapy vectors based on either previous studies; Drd1a, Drd2 and pDyn, or from a microarray study on parkinsonian patients; ACE, DNAJC3, GALNS, MAP1a and RNF25. These candidates have been evaluated in rat striatum to determine their suitability for use in cell specific vectors. The promoters had a neuronal specificity of 91-100%. The efficiency of the promoters was variable, but RNF25, DNAJC3 and MAP1a were comparable to widely used ubiquitous promoters. MAP1a was also affected by dopamine depletion.

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Authors
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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences

Keywords

  • Promoter, Parkinson's disease, Gene therapy, Neuron-specific
Original languageEnglish
Pages (from-to)29-34
JournalNeuroscience Letters
Volume530
Issue number1
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes

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