Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection

Research output: Contribution to journalArticle

Abstract

A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.

Details

Authors
  • Ibolya Bontidean
  • Josefin Ahlqvist
  • Ashok Mulchandani
  • Wilfred Chen
  • Weon Bae
  • Rajesh K Mehra
  • Alessia Mortari
  • Elisabeth Csöregi
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • Biosensors, Heavy metals, Phytochelatins, Capacitance
Original languageEnglish
Pages (from-to)547-553
JournalBiosensors & Bioelectronics
Volume18
Issue number5-6
Publication statusPublished - 2003
Publication categoryResearch
Peer-reviewedYes