Nucleolar proteins with altered expression in leukemic cell lines.

Research output: Contribution to journalArticle

Abstract

The function of the nucleolus is intimately connected to cell proliferation, division and growth. Many cancer cells have enlarged nucleoli, and several nucleolar proteins have been linked to tumorigenesis. In order to find proteins whose expression is altered in the nucleoli of leukemic cells, we carried out two-dimensional difference gel electrophoresis (2-D DIGE) analyses. Prohibitin (PHB) and TAR-DNA-binding protein-43 (TDP-43) were strongly expressed in the nucleoli of the pre-B-ALL cell line MHH-CALL3. Our results demonstrate that leukemic cells have differences in their nucleolar protein composition, and suggest that it may be possible to exploit these differences in identification of leukemia subtypes.

Details

Authors
  • Kaisa J Teittinen
  • Pauliina Kärkkäinen
  • Johanna Salonen
  • Gunilla Rönnholm
  • Hanna Korkeamäki
  • Mauno Vihinen
  • Nisse Kalkkinen
  • Olli Lohi
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology

Keywords

  • Repressor Proteins: metabolism, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma: metabolism, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma: diagnosis, Nuclear Proteins: metabolism, Cell Nucleolus: metabolism, DNA-Binding Proteins: metabolism, Tumor Markers, Biological: metabolism
Original languageEnglish
Pages (from-to)232-236
JournalLeukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis
Volume36
Issue number2
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes