On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes

Research output: Contribution to journalArticle


An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perfusion liquid used for sampling hydrophobic eicosanoids in biological systems was also studied. The total time for one analysis was 7.6 min allowing seven measurements per hour for monitoring kinetic changes in biological systems.


External organisations
  • Astra Draco AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry


  • Autoanalysis, Cell Line, Chromatography, Liquid/instrumentation, Culture Media, Conditioned, Cyclodextrins, Eicosanoids/analysis, Humans, Hydrogen-Ion Concentration, Indicators and Reagents, Leukotriene B4/analysis, Leukotriene C4/analysis, Leukotriene D4/analysis, Leukotriene E4/analysis, Leukotrienes/analysis, Microdialysis/instrumentation, Monocytes/metabolism, Prostaglandins B/analysis
Original languageEnglish
Pages (from-to)489-496
Number of pages8
JournalJournal of chromatography. A
Issue number1-2
Publication statusPublished - 1998 Oct 9
Publication categoryResearch