On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes

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On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes. / Kjellström, S; Emnéus, J; Laurell, T; Heintz, L; Marko-Varga, G.

In: Journal of chromatography. A, Vol. 823, No. 1-2, 09.10.1998, p. 489-496.

Research output: Contribution to journalArticle

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TY - JOUR

T1 - On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes

AU - Kjellström, S

AU - Emnéus, J

AU - Laurell, T

AU - Heintz, L

AU - Marko-Varga, G

PY - 1998/10/9

Y1 - 1998/10/9

N2 - An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perfusion liquid used for sampling hydrophobic eicosanoids in biological systems was also studied. The total time for one analysis was 7.6 min allowing seven measurements per hour for monitoring kinetic changes in biological systems.

AB - An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perfusion liquid used for sampling hydrophobic eicosanoids in biological systems was also studied. The total time for one analysis was 7.6 min allowing seven measurements per hour for monitoring kinetic changes in biological systems.

KW - Autoanalysis

KW - Cell Line

KW - Chromatography, Liquid/instrumentation

KW - Culture Media, Conditioned

KW - Cyclodextrins

KW - Eicosanoids/analysis

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Indicators and Reagents

KW - Leukotriene B4/analysis

KW - Leukotriene C4/analysis

KW - Leukotriene D4/analysis

KW - Leukotriene E4/analysis

KW - Leukotrienes/analysis

KW - Microdialysis/instrumentation

KW - Monocytes/metabolism

KW - Prostaglandins B/analysis

U2 - 10.1016/S0021-9673(98)00361-6

DO - 10.1016/S0021-9673(98)00361-6

M3 - Article

VL - 823

SP - 489

EP - 496

JO - Journal of Chromatography

T2 - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1-2

ER -