Optical projection tomography for rapid whole mouse brain imaging

Research output: Contribution to journalArticle


In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.


External organisations
  • Swiss Federal Institute of Technology
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Radiology, Nuclear Medicine and Medical Imaging
Original languageEnglish
Article number#301816
Pages (from-to)5637-5650
Number of pages14
JournalBiomedical Optics Express
Issue number12
Publication statusPublished - 2017 Dec 1
Publication categoryResearch

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