Optimization of integrated chromatography sequences for purification of biopharmaceuticals

Research output: Contribution to journalArticle

Abstract

With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a four-column integrated sequence and a continuously loaded twin-capture setup with five columns.

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Authors
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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Pharmaceutical Chemistry

Keywords

  • chromatography, continuous bioprocessing, integrated column sequences, multiple column design, optimization
Original languageEnglish
Article numbere2871
JournalBiotechnology Progress
Volume35
Issue number6
Early online date2019 Jun 17
Publication statusPublished - 2019 Nov
Publication categoryResearch
Peer-reviewedYes