Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow

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Bibtex

@article{f06f314ef1444c7d92c043ca4c548fe1,
title = "Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow",
abstract = "The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C. The yield decreased with increasing temperature, due to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures.",
author = "R Eklund and Mats Galbe and Guido Zacchi",
year = "1990",
doi = "10.1016/0141-0229(90)90043-P",
language = "English",
volume = "12",
pages = "225--228",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier",
number = "3",

}