Oxidative metabolism of dopamine: a colour reaction from human midbrain analysed by mass spectrometry

Research output: Contribution to specialist publication or newspaperSpecialist publication article

Abstract

In order to identify the protein responsible for a dopamine peroxidizing activity, previously described in human normal and parkinsonian substantia nigra by our group, we developed non-denaturing polyacrylamide gel electrophoresis conditions, mimicking the characteristic colour in vitro reaction, resulting from cyclic oxidation of dopamine (DA). After separating protein mixtures from human normal midbrain homogenates on two sets of identical native gels, one gel set was subjected to specific activity staining by using DA and hydrogen peroxide. An activity red/orange band appeared in midbrain tissue lanes, similarly to the lane where commercial horseradish peroxidase (HRP) was present as control of peroxidative activity. The second set of gels, stained with Coomassie Blue, showed other, not enzymatically active protein bands. Mass spectrometry analysis of the bands containing the activity and the corresponding Coomassie Blue bands revealed the presence of proteins that may play a role in neurodegenerative disease, highlighting a possible functional link among dopamine/dopaminochrome redox cycle and protein metabolism.

Details

Authors
  • A De Iuliis
  • Giorgio Arrigoni
  • L Andersson
  • P Zambenedetti
  • A Burlina
  • Peter James
  • P Arslan
  • F Vianello
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area
Original languageEnglish
Pages1687-1693
Volume1784
Specialist publication or newspaperBiochimica et Biophysica Acta - Proteins and Proteomics
Publication statusPublished - 2008
Publication categoryPopular science