Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring

Research output: Contribution to journalArticle

Abstract

Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency,I-max, K-M(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H2O2. The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP.

Details

Authors
  • IS Alpeeva
  • Mihaela Nistor
  • Jaime Castillo Leon
  • Elisabeth Csöregi
  • IY Sakharova
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry

Keywords

  • sweet potato, horseradish, palm tree, peroxidase, biosensors, hydrogen, peroxide
Original languageEnglish
Pages (from-to)742-748
JournalBiosensors & Bioelectronics
Volume21
Issue number5
Publication statusPublished - 2005
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)