Patient-tailored analysis of minimal residual disease in acute myeloid leukemia using next-generation sequencing

Research output: Contribution to journalArticle

Abstract

Next-generation sequencing techniques have revealed that leukemic cells in acute myeloid leukemia often are characterized by a limited number of somatic mutations. These mutations can be the basis for the detection of leukemic cells in follow-up samples. The aim of this study was to identify leukemia-specific mutations in cells from patients with acute myeloid leukemia and to use these mutations as markers for minimal residual disease. Leukemic cells and normal lymphocytes were simultaneously isolated at diagnosis from 17 patients with acute myeloid leukemia using fluorescence-activated cell sorting. Exome sequencing of these cells identified 240 leukemia-specific single nucleotide variations and 22 small insertions and deletions. Based on estimated allele frequencies and their accuracies, 191 of these mutations qualified as candidates for minimal residual disease analysis. Targeted deep sequencing with a significance threshold of 0.027% for single nucleotide variations and 0.006% for NPM1 type A mutation was developed for quantification of minimal residual disease. When tested on follow-up samples from a patient with acute myeloid leukemia, targeted deep sequencing of single nucleotide variations as well as NPM1 was more sensitive than minimal residual disease quantification with multiparameter flow cytometry. In conclusion, we here describe how exome sequencing can be used for identification of leukemia-specific mutations in samples already at diagnosis of acute myeloid leukemia. We also show that targeted deep sequencing of such mutations, including single nucleotide variations, can be used for high-sensitivity quantification of minimal residual disease in a patient-tailored manner.

Details

Authors
  • Erik B R Malmberg
  • Sara Ståhlman
  • Anna Rehammar
  • Tore Samuelsson
  • Sofie J. Alm
  • Erik Kristiansson
  • Jonas Abrahamsson
  • Hege Garelius
  • Louise Pettersson
  • Mats Ehinger
  • Lars Palmqvist
  • Linda Fogelstrand
Organisations
External organisations
  • University of Gothenburg
  • Chalmers University of Technology
  • Sahlgrenska University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology
  • Cell and Molecular Biology

Keywords

  • Acute myeloid leukemia, Massively parallel sequencing, Minimal residual disease
Original languageEnglish
Pages (from-to)26-37
JournalEuropean Journal of Haematology
Volume98
Issue number1
Early online date2016 May 20
Publication statusPublished - 2017 Jan
Publication categoryResearch
Peer-reviewedYes