PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera

Research output: Contribution to journalArticle

Abstract

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.

Details

Authors
Organisations
External organisations
  • Stockholm University
  • University of Turku
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Zoology
  • Biochemistry and Molecular Biology
  • Genetics

Keywords

  • Lepidoptera, Molecular systematics, Phylogenetics, Phylogenomics
Original languageEnglish
Pages (from-to)129-141
Number of pages13
JournalZooKeys
Volume2016
Issue number596
Publication statusPublished - 2016
Publication categoryResearch
Peer-reviewedYes