PDGF-B Is Required for Development of the Glymphatic System

Research output: Contribution to journalArticle

Abstract

The glymphatic system is a highly polarized cerebrospinal fluid (CSF) transport system that facilitates the clearance of neurotoxic molecules through a brain-wide network of perivascular pathways. Herein we have mapped the development of the glymphatic system in mice. Perivascular CSF transport first emerges in hippocampus in newborn mice, and a mature glymphatic system is established in the cortex at 2 weeks of age. Formation of astrocytic endfeet and polarized expression of aquaporin 4 (AQP4) consistently coincided with the appearance of perivascular CSF transport. Deficiency of platelet-derived growth factor B (PDGF-B) function in the PDGF retention motif knockout mouse line Pdgfb ret/ret suppressed the development of the glymphatic system, whose functions remained suppressed in adulthood compared with wild-type mice. These experiments map the natural development of the glymphatic system in mice and define a critical role of PDGF-B in the development of perivascular CSF transport.

Details

Authors
Organisations
External organisations
  • University of Rochester
  • University of Copenhagen
  • Uppsala University
  • Karolinska Institutet
  • Mansoura University
  • Mansoura University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology

Keywords

  • aquaporin 4, astrocytes, cerebrospinal fluid, glymphatic system, platelet-derived growth factor B, postnatal development
Original languageEnglish
Pages (from-to)2955-2969.e3
JournalCell Reports
Publication statusPublished - 2019
Publication categoryResearch
Peer-reviewedYes