Penetration of aluminium salts through pig skin in vitro

Research output: Contribution to journalPublished meeting abstract

Abstract

Background: Recently it was suggested to use aluminium chloride hexahydrate in petrolatum at 10% to detect aluminium contact allergy. Patch testing with aluminium chloride hexahydrate at 20% did not demonstrate a higher number of positive test reactions. It was speculated that the constricting effect of aluminium salts might impair skin penetration. Objectives: The purpose of the present study is to estimate the concentration of aluminium in various skin layers and in the recipient phase using different aluminium test preparations on pig skin in an in vitro diffusion cell system. Methods: Full-thickness (7sim; porcine ear skin was mounted in Franz-type diffusion cells. The diffusional area was 0.64 cm2 and recipient volume 4 ml. The dermal side was exposed to a recipient solution consisting of PBS (phosphate buffer saline, pH 7.4). Aluminium chloride hexahydrate preparations in petrolatum and in water at 10% were applied to the donor compartment facing the epidermis for 24 hr. All samples were analysed for total aluminium by atomic absorption spectrometry. The limit of detection was 0.01 μg/ml. Results: No aluminium could be detected in the recipient phase in the six diffusion cells. Conclusions: Neither aluminium in petrolatum nor in water gave detectable amounts of penetrating aluminium in 24 h. The lipophilicity of the vehicle was not decisive.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Dermatology and Venereal Diseases
  • Respiratory Medicine and Allergy

Keywords

  • aluminum salt, aluminum, petrolatum, aluminum chloride, water, sodium chloride, phosphate, pig, skin, in vitro study, society, contact dermatitis, recipient, diffusion, lipophilicity, atomic absorption spectrometry, epidermis, skin penetration, donor, pH, limit of detection, ear, thickness, contact allergy
Original languageEnglish
Pages (from-to)52
JournalContact Dermatitis
Volume66
Issue numbers2
Publication statusPublished - 2012 Jun 1
Publication categoryResearch
Peer-reviewedNo
Event11th Congress of the European Society of Contact Dermatitis (ESCD) - Malmö, Sweden
Duration: 2012 Jun 132012 Jun 16