Peptides analysis in blood plasma using on-line system of supported liquid membrane and high-performance liquid chromatography

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Abstract

The potential of using supported liquid membrane (SLM) technique, combined with reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated for the determination of peptides in human blood plasma. The peptides studied were (DL)Leu(DL)Phe, MetLeuPhe, GlyLeuTyr and ValGluProlleProTyr. The carrier (Aliquat 336) was incorporated in membrane phase in order to facilitate the transport of investigated peptides. After extraction, the analyte-enriched acceptor phase was directly injected into an HPLC system for analysis. With SLM, high selectivity and efficiency were achieved for extraction of peptides in aqueous solutions. Lower extraction efficiency was obtained in plasma sample. The limiting factors were the presence of salts and proteins in blood plasma. The necessary of ultrafiltration application before the SLM was required in order to improve its stability. The minimum quantifiable concentration was 90-130 ng/ml of the investigated peptides based on 0.5 ml plasma. The standard curve was linear over the concentration range of 0.05-10 mu g/ml. (c) 2005 Elsevier B.V. All rights reserved.

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  • Analytical Chemistry
Original languageEnglish
Pages (from-to)9-14
JournalAnalytica Chimica Acta
Volume553
Issue number1-2
Publication statusPublished - 2005
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)