Permeability of parietal pleura to liquid and proteins

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Permeability of parietal pleura to liquid and proteins. / Negrini, D; Venturoli, Daniele; Townsley, M I; Reed, R K.

In: Journal of Applied Physiology, Vol. 76, No. 2, 1994, p. 627-633.

Research output: Contribution to journalArticle

Harvard

Negrini, D, Venturoli, D, Townsley, MI & Reed, RK 1994, 'Permeability of parietal pleura to liquid and proteins', Journal of Applied Physiology, vol. 76, no. 2, pp. 627-633.

APA

Negrini, D., Venturoli, D., Townsley, M. I., & Reed, R. K. (1994). Permeability of parietal pleura to liquid and proteins. Journal of Applied Physiology, 76(2), 627-633.

CBE

Negrini D, Venturoli D, Townsley MI, Reed RK. 1994. Permeability of parietal pleura to liquid and proteins. Journal of Applied Physiology. 76(2):627-633.

MLA

Vancouver

Negrini D, Venturoli D, Townsley MI, Reed RK. Permeability of parietal pleura to liquid and proteins. Journal of Applied Physiology. 1994;76(2):627-633.

Author

Negrini, D ; Venturoli, Daniele ; Townsley, M I ; Reed, R K. / Permeability of parietal pleura to liquid and proteins. In: Journal of Applied Physiology. 1994 ; Vol. 76, No. 2. pp. 627-633.

RIS

TY - JOUR

T1 - Permeability of parietal pleura to liquid and proteins

AU - Negrini, D

AU - Venturoli, Daniele

AU - Townsley, M I

AU - Reed, R K

PY - 1994

Y1 - 1994

N2 - The permselectivity of the parietal pleura was determined in spontaneously breathing anesthetized rabbits and dogs. In rabbits, we injected intrapleurally 5 ml of 1-g/dl albumin solution containing 100 microCi of 131I-labeled albumin plus 100 microCi of either lactate dehydrogenase (LDH) or alpha 2-125I-macroglobulin. Dogs received 100 ml of 1-g/dl albumin solution containing 100 microCi of 131I-albumin plus 100 microCi of alpha 2-125I-macroglobulin. A transpleural pressure gradient was set, lowering the intracapsular pressure to -30 cmH2O. The solvent drag reflection coefficients (sigma f) were calculated as the ratio between tracer concentrations in capsular and pleural liquid collected at 60-180 min. In rabbits sigma f was 0.44 +/- 0.2 (SD) for albumin, 0.84 +/- 0.1 for LDH, and 0.93 +/- 0.05 for alpha 2-macroglobulin. In dogs sigma f was 0.30 +/- 0.19 for albumin and 0.53 +/- 0.15 for alpha 2-macroglobulin. The hydraulic conductivity of the parietal pleura was 2.18 +/- 1.54 microliters.h-1.cmH2O-1.cm-2 in rabbits and 1.22 +/- 1.13 microliters.h-1.cmH2O-1.cm-2 in dogs. The parietal pleura could be modeled by two pore populations with radii of 83-89 and 156-222 A. The permeability coefficient averaged 0.08-0.21 x 10(-6) cm/s for albumin, 0.06-0.09 x 10(-6) cm/s for LDH, and 0.01-0.03 x 10(-6) cm/s for alpha 2-macroglobulin.

AB - The permselectivity of the parietal pleura was determined in spontaneously breathing anesthetized rabbits and dogs. In rabbits, we injected intrapleurally 5 ml of 1-g/dl albumin solution containing 100 microCi of 131I-labeled albumin plus 100 microCi of either lactate dehydrogenase (LDH) or alpha 2-125I-macroglobulin. Dogs received 100 ml of 1-g/dl albumin solution containing 100 microCi of 131I-albumin plus 100 microCi of alpha 2-125I-macroglobulin. A transpleural pressure gradient was set, lowering the intracapsular pressure to -30 cmH2O. The solvent drag reflection coefficients (sigma f) were calculated as the ratio between tracer concentrations in capsular and pleural liquid collected at 60-180 min. In rabbits sigma f was 0.44 +/- 0.2 (SD) for albumin, 0.84 +/- 0.1 for LDH, and 0.93 +/- 0.05 for alpha 2-macroglobulin. In dogs sigma f was 0.30 +/- 0.19 for albumin and 0.53 +/- 0.15 for alpha 2-macroglobulin. The hydraulic conductivity of the parietal pleura was 2.18 +/- 1.54 microliters.h-1.cmH2O-1.cm-2 in rabbits and 1.22 +/- 1.13 microliters.h-1.cmH2O-1.cm-2 in dogs. The parietal pleura could be modeled by two pore populations with radii of 83-89 and 156-222 A. The permeability coefficient averaged 0.08-0.21 x 10(-6) cm/s for albumin, 0.06-0.09 x 10(-6) cm/s for LDH, and 0.01-0.03 x 10(-6) cm/s for alpha 2-macroglobulin.

M3 - Article

VL - 76

SP - 627

EP - 633

JO - Journal of Applied Physiology

T2 - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 1522-1601

IS - 2

ER -