Perspectives of targeted mass spectrometry for protein biomarker verification

Research output: Contribution to journalArticle

Abstract

The identification of specific biomarkers will improve the early diagnosis of disease, facilitate the development of targeted therapies, and provide an accurate method to monitor treatment response. A major challenge in the process of verifying biomarker candidates in blood plasma is the complexity and high dynamic range of proteins. This article reviews the current, targeted proteomic strategies that are capable of quantifying biomarker candidates at concentration ranges where biomarkers are expected in plasma (i.e. at the ng/ml level). In addition, a workflow is presented that allows the fast and definitive generation of targeted mass spectrometry-based assays for most biomarker candidate proteins. These assays are stored in publicly accessible databases and have the potential to greatly impact the throughput of biomarker verification studies.

Details

Authors
External organisations
  • ETH Zürich
Research areas and keywords

Keywords

  • Animals, Biomarkers, Blood Proteins, Databases, Protein, Humans, Mass Spectrometry, Proteins, Proteomics, Reproducibility of Results, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review
Original languageEnglish
Pages (from-to)518-25
Number of pages8
JournalCurrent Opinion in Chemical Biology
Volume13
Issue number5-6
Publication statusPublished - 2009 Dec
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes