Phosphite-induced changes of the transcriptome and secretome in Solanum tuberosum leading to resistance against Phytophthora infestans

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Phosphite-induced changes of the transcriptome and secretome in Solanum tuberosum leading to resistance against Phytophthora infestans. / Burra, Dharani Dhar; Berkowitz, Oliver; Hedley, Pete E.; Morris, Jenny; Resjoe, Svante; Levander, Fredrik; Liljeroth, Erland; Andreasson, Erik; Alexandersson, Erik.

In: BMC Plant Biology, Vol. 14, 254, 2014.

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Burra, Dharani Dhar ; Berkowitz, Oliver ; Hedley, Pete E. ; Morris, Jenny ; Resjoe, Svante ; Levander, Fredrik ; Liljeroth, Erland ; Andreasson, Erik ; Alexandersson, Erik. / Phosphite-induced changes of the transcriptome and secretome in Solanum tuberosum leading to resistance against Phytophthora infestans. In: BMC Plant Biology. 2014 ; Vol. 14.

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TY - JOUR

T1 - Phosphite-induced changes of the transcriptome and secretome in Solanum tuberosum leading to resistance against Phytophthora infestans

AU - Burra, Dharani Dhar

AU - Berkowitz, Oliver

AU - Hedley, Pete E.

AU - Morris, Jenny

AU - Resjoe, Svante

AU - Levander, Fredrik

AU - Liljeroth, Erland

AU - Andreasson, Erik

AU - Alexandersson, Erik

PY - 2014

Y1 - 2014

N2 - Background: Potato late blight caused by the oomycete pathogen Phytophthora infestans can lead to immense yield loss. We investigated the transcriptome of Solanum tubersoum (cv. Desiree) and characterized the secretome by quantitative proteomics after foliar application of the protective agent phosphite. We also studied the distribution of phosphite in planta after application and tested transgenic potato lines with impaired in salicylic and jasmonic acid signaling. Results: Phosphite had a rapid and transient effect on the transcriptome, with a clear response 3 h after treatment. Strikingly this effect lasted less than 24 h, whereas protection was observed throughout all time points tested. In contrast, 67 secretome proteins predominantly associated with cell-wall processes and defense changed in abundance at 48 h after treatment. Transcripts associated with defense, wounding, and oxidative stress constituted the core of the phosphite response. We also observed changes in primary metabolism and cell wall-related processes. These changes were shown not to be due to phosphate depletion or acidification caused by phosphite treatment. Of the phosphite-regulated transcripts 40% also changed with beta-aminobutyric acid (BABA) as an elicitor, while the defence gene PR1 was only up-regulated by BABA. Although phosphite was shown to be distributed in planta to parts not directly exposed to phosphite, no protection in leaves without direct foliar application was observed. Furthermore, the analysis of transgenic potato lines indicated that the phosphite-mediated resistance was independent of the plant hormones salicylic and jasmonic acid. Conclusions: Our study suggests that a rapid phosphite-triggered response is important to confer long-lasting resistance against P. infestans and gives molecular understanding of its successful field applications.

AB - Background: Potato late blight caused by the oomycete pathogen Phytophthora infestans can lead to immense yield loss. We investigated the transcriptome of Solanum tubersoum (cv. Desiree) and characterized the secretome by quantitative proteomics after foliar application of the protective agent phosphite. We also studied the distribution of phosphite in planta after application and tested transgenic potato lines with impaired in salicylic and jasmonic acid signaling. Results: Phosphite had a rapid and transient effect on the transcriptome, with a clear response 3 h after treatment. Strikingly this effect lasted less than 24 h, whereas protection was observed throughout all time points tested. In contrast, 67 secretome proteins predominantly associated with cell-wall processes and defense changed in abundance at 48 h after treatment. Transcripts associated with defense, wounding, and oxidative stress constituted the core of the phosphite response. We also observed changes in primary metabolism and cell wall-related processes. These changes were shown not to be due to phosphate depletion or acidification caused by phosphite treatment. Of the phosphite-regulated transcripts 40% also changed with beta-aminobutyric acid (BABA) as an elicitor, while the defence gene PR1 was only up-regulated by BABA. Although phosphite was shown to be distributed in planta to parts not directly exposed to phosphite, no protection in leaves without direct foliar application was observed. Furthermore, the analysis of transgenic potato lines indicated that the phosphite-mediated resistance was independent of the plant hormones salicylic and jasmonic acid. Conclusions: Our study suggests that a rapid phosphite-triggered response is important to confer long-lasting resistance against P. infestans and gives molecular understanding of its successful field applications.

KW - Phosphite

KW - Late blight

KW - Phytophthora infestans

KW - Potato

KW - Secretome

KW - Microarray

KW - Induced resistance

KW - Transgenic lines

U2 - 10.1186/s12870-014-0254-y

DO - 10.1186/s12870-014-0254-y

M3 - Article

VL - 14

JO - BMC Plant Biology

T2 - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

M1 - 254

ER -