Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium

Research output: Contribution to journalArticle

Abstract

Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g CDW h) −1 respectively. The isolate was further subjected to whole genome sequencing and putative genes related to the metabolism of syringyl-compounds were mapped for the first time in a Gram-positive bacterium. These findings will be of high significance when designing future host microorganisms and bioprocesses for the efficient valorization of pre-treated lignin feedstocks.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Bioprocess Technology

Keywords

  • Gram-positive, Lignin bioconversion, Microbacterium, Syringaldehyde, Syringate demethylase
Original languageEnglish
Article number121327
JournalBioresource Technology
Volume285
Publication statusPublished - 2019
Publication categoryResearch
Peer-reviewedYes

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