Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium

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Bibtex

@article{03a904292a6d4497ad223fd04655800e,
title = "Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium",
abstract = " Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g CDW h) −1 respectively. The isolate was further subjected to whole genome sequencing and putative genes related to the metabolism of syringyl-compounds were mapped for the first time in a Gram-positive bacterium. These findings will be of high significance when designing future host microorganisms and bioprocesses for the efficient valorization of pre-treated lignin feedstocks. ",
keywords = "Gram-positive, Lignin bioconversion, Microbacterium, Syringaldehyde, Syringate demethylase",
author = "Krithika Ravi and Javier Garc{\'i}a-Hidalgo and Brink, {Daniel P.} and Martin Skyvell and Gorwa-Grauslund, {Marie F.} and Gunnar Lid{\'e}n",
year = "2019",
doi = "10.1016/j.biortech.2019.121327",
language = "English",
volume = "285",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}