Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium

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T1 - Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium

AU - Ravi, Krithika

AU - García-Hidalgo, Javier

AU - Brink, Daniel P.

AU - Skyvell, Martin

AU - Gorwa-Grauslund, Marie F.

AU - Lidén, Gunnar

PY - 2019

Y1 - 2019

N2 - Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g CDW h) −1 respectively. The isolate was further subjected to whole genome sequencing and putative genes related to the metabolism of syringyl-compounds were mapped for the first time in a Gram-positive bacterium. These findings will be of high significance when designing future host microorganisms and bioprocesses for the efficient valorization of pre-treated lignin feedstocks.

AB - Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g CDW h) −1 respectively. The isolate was further subjected to whole genome sequencing and putative genes related to the metabolism of syringyl-compounds were mapped for the first time in a Gram-positive bacterium. These findings will be of high significance when designing future host microorganisms and bioprocesses for the efficient valorization of pre-treated lignin feedstocks.

KW - Gram-positive

KW - Lignin bioconversion

KW - Microbacterium

KW - Syringaldehyde

KW - Syringate demethylase

U2 - 10.1016/j.biortech.2019.121327

DO - 10.1016/j.biortech.2019.121327

M3 - Article

C2 - 30991184

AN - SCOPUS:85064121207

VL - 285

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

M1 - 121327

ER -