Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture.

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Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture. / Quillaguaman, Jorge; Doan Van, Thuoc; Guzmán, Hector; Guzmán, Daniel; Martín, Javier; Everest, Akaraonye; Hatti-Kaul, Rajni.

In: Applied Microbiology and Biotechnology, Vol. 78, No. 2, 2008, p. 227-232.

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Quillaguaman, J, Doan Van, T, Guzmán, H, Guzmán, D, Martín, J, Everest, A & Hatti-Kaul, R 2008, 'Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture.', Applied Microbiology and Biotechnology, vol. 78, no. 2, pp. 227-232. https://doi.org/10.1007/s00253-007-1297-x

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Quillaguaman, Jorge ; Doan Van, Thuoc ; Guzmán, Hector ; Guzmán, Daniel ; Martín, Javier ; Everest, Akaraonye ; Hatti-Kaul, Rajni. / Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture. In: Applied Microbiology and Biotechnology. 2008 ; Vol. 78, No. 2. pp. 227-232.

RIS

TY - JOUR

T1 - Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture.

AU - Quillaguaman, Jorge

AU - Doan Van, Thuoc

AU - Guzmán, Hector

AU - Guzmán, Daniel

AU - Martín, Javier

AU - Everest, Akaraonye

AU - Hatti-Kaul, Rajni

PY - 2008

Y1 - 2008

N2 - High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH(4)Cl and 0.22% (w/v) K(2)HPO(4) and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l(-1), respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH(4)Cl, and K(2)HPO(4) were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l(-1), PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l(-1) h(-1).

AB - High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH(4)Cl and 0.22% (w/v) K(2)HPO(4) and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l(-1), respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH(4)Cl, and K(2)HPO(4) were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l(-1), PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l(-1) h(-1).

U2 - 10.1007/s00253-007-1297-x

DO - 10.1007/s00253-007-1297-x

M3 - Article

VL - 78

SP - 227

EP - 232

JO - European Journal of Applied Microbiology and Biotechnology

T2 - European Journal of Applied Microbiology and Biotechnology

JF - European Journal of Applied Microbiology and Biotechnology

SN - 1432-0614

IS - 2

ER -