Polyclonal expansion of cells with trisomy 7 in synovia from patients with osteoarthritis

Research output: Contribution to journalArticle

Abstract

Trisomy 7 as the single chromosome aberration has been found in a variety of neoplasms and in normal tissue in the proximity of tumors, as well as in non-neoplastic lesions. Recently, we described a nonrandom pattern of chromosome aberrations, in particular, a gain of chromosome 7, in synovia, cartilage, and osteophytes from patients with osteoarthritis. To study the clonal origin of trisomy 7 in osteoarthritis, multiple synovial samples were collected from five women, all of whom were informative heterozygotes with regard to the X-linked human androgen receptor gene (AR). From each case, three to four independent cell cultures were initiated. Trisomic cell populations were subcloned from the individual cultures, and it was established whether or not the same allele of AR was inactivated in trisomic cells from different parts of the same joint. The finding of a polyclonal X-inactivation pattern in two of the cases provides strong evidence that gain of an extra copy of chromosome 7 occurs independently in multiple cells.

Details

Authors
External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Genetics

Keywords

  • Aged, Biopsy, Chromosome Aberrations, Chromosomes, Human, Pair 7, Cloning, Molecular, Dosage Compensation, Genetic, Female, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Osteoarthritis/genetics, Synovial Membrane/pathology, Trisomy/genetics
Original languageEnglish
Pages (from-to)30-4
JournalCytogenetics and Cell Genetics
Volume83
Issue number1-2
Publication statusPublished - 1998
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes