Polymerase chain reaction for detection of Mycobacterium tuberculosis

Research output: Contribution to journalArticle

Abstract

A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.

Details

Authors
  • Ulf Sjöbring
  • Michael Mecklenburg
  • Åse Bengård Andersen
  • Håkan Miörner
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area
Original languageEnglish
Pages (from-to)2200-2204
JournalJournal of Clinical Microbiology
Volume28
Issue number10
Publication statusPublished - 1990
Publication categoryResearch
Peer-reviewedYes