Polymorphisms in glutathione-related genes modify mercury concentrations and antioxidant status in subjects environmentally exposed to methylmercury

Research output: Contribution to journalArticle

Abstract

Methylmercury (MeHg) toxicity may vary widely despite similar levels of exposure. This is hypothetically related to genetic differences in enzymes metabolizing MeHg. MeHg causes oxidative stress in experimental models but little is known about its effects on humans. The aims of the present study was to evaluate the effects of polymorphisms in glutathione (GSH)-related genes (GSTM1, GSTT1, GSTP1 and GCLM) on Hg concentrations in blood and hair, as well as MeHg-related effects on catalase (CAT) and glutathione-peroxidase (GPx) activity and GSH concentrations. Study subjects were from an Amazonian population in Brazil chronically exposed to MeHg from fish. Hg in blood and hair were determined by ICP-MS, CAT, GPx and GSH were determined by spectrophotometry, and multiplex PCR (GSTM1 and GSTT1) and TagMan assays (GSTP1 and GCLM) were used for genotyping. Mean Hg concentrations in blood and hair were 48 +/- 36 mu g/L and 14 +/- 10 mu g/g. Persons with the GCLM-588 IT genotype had lower blood and hair Hg than did C-allele carriers (linear regression for Hg in blood beta = -0.32, p = 0.017; and hair beta = -0.33; p = 0.0090; adjusted for fish intake, age and gender). GSTM1*0 homozygous had higher blood (beta = 0.20; p = 0.017) and hair Hg (hair beta = 0.20; p = 0.013). Exposure to MeHg altered antioxidant status (CAT:beta = -0.086; GSH:beta = -0.12; GPx:beta = -0.16; all p < 0.010; adjusted for gender, age and smoking). Persons with GSTM1*0 had higher CAT activity in the blood than those with GSTM1. Our data thus indicate that some GSH-related polymorphisms, such as GSTM1 and GCLM may modify MeHg metabolism and Hg-related antioxidant effects. (C) 2013 Elsevier BY. All rights reserved.

Details

Authors
  • Gustavo Rafael Mazzaron Barcelos
  • Denise Grotto
  • Katia Cristina de Marco
  • Juliana Valentini
  • Andre van Helvoort Lengert
  • Andreia Avila Soares de Oliveira
  • Solange Cristina Garcia
  • Gilberto Ubida Leite Braga
  • Karin Engström
  • Ilce Mara de Syllos Colus
  • Karin Broberg Palmgren
  • Fernando Jr. Barbosa
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Environmental Sciences

Keywords

  • Antioxidant status, Fish intake, Gene-environment interactions, Metabolism, Methylmercury, Polymorphisms
Original languageEnglish
Pages (from-to)319-325
JournalScience of the Total Environment
Volume463
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes