Polypoidal Choroidal Vasculopathy Associate With Diminished Regulatory T Cells That Are Polarized Into a T Helper 2-Like Phenotype

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@article{21ff4e7a90134bcfabd03b505eeaacb1,
title = "Polypoidal Choroidal Vasculopathy Associate With Diminished Regulatory T Cells That Are Polarized Into a T Helper 2-Like Phenotype",
abstract = "Purpose: To investigate possible roles of T helper (Th) cells, regulatory T cells (Tregs), and the recently mapped Th-like Tregs in patients with polypoidal choroidal vasculopathy (PCV). Methods: In this prospective case-control study, we obtained fresh venous blood from patients with PCV (n = 24), age-matched healthy controls (n = 32), and patients with neovascular AMD (n = 45). All participants underwent a comprehensive ocular examination including fluorescein and indocyanine green angiography for where retinal disease was suspected. Using flow cytometry, we identified Th subsets, Tregs, and Th-like Tregs. Plasma samples were stored at -80°C to investigate plasma cytokines of interest. Results: Compared to healthy controls, patients with PCV had lower percentages of Tregs (8.7{\%} ± 2.8{\%} vs. 7.3{\%} ± 1.7{\%}, P = 0.027), which were significantly more Th2-like polarized (42.6{\%} ± 13.3{\%} vs. 50.5{\%} ± 13.0{\%}, P = 0.029). These changes differed from that observed in neovascular AMD, which compared to healthy controls had fewer Th1/Th17 cells (3.6{\%} ± 2.7{\%} vs. 2.4{\%} ± 2.5{\%}, P = 0.049), comparable Treg levels, and no distinct polarization of Th-like Tregs. Because of these findings, we measured plasma IL-4 and IL-33 levels. Plasma IL-33 in patients with PCV (median 0.30 pg/mL) was twice as high compared to healthy controls (median 0.16 pg/mL; P = 0.037). Conclusions: PCV associate with diminished Tregs that are polarized more into a Th2-like phenotype. This is correlated to IL-33 levels, which we also find increased in patients with PCV. Our findings suggest a possible role for Th2-like Tregs and IL-33 in PCV.",
author = "Yousif Subhi and {Krogh Nielsen}, Marie and Molbech, {Christopher Rue} and Akio Oishi and Amardeep Singh and Nissen, {Mogens Holst} and S{\o}rensen, {Torben Lykke}",
year = "2019",
month = "6",
day = "3",
doi = "10.1167/iovs.19-26882",
language = "English",
volume = "60",
pages = "2583--2590",
journal = "Investigative Ophthalmology & Visual Science",
issn = "1552-5783",
publisher = "ASSOC RESEARCH VISION OPHTHALMOLOGY INC",
number = "7",

}