Porous silicon microarray for simultaneous fluorometric immunoassay of the biomarkers prostate-specific antigen and human glandular kallikrein 2

Research output: Contribution to journalArticle


The authors have developed a porous silicon (P-Si) based duplex antibody microarray platform for simultaneous quantitation of the biomarkers prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) in serum. Pore size-controlled P-Si surfaces have an extremely enlarged surface area that enables high-density immobilization of fluorescently labeled antibodies by physical adsorption. Automated microarraying of the antibodies provides a fast and reproducible duplex format of antibody arrays on the P-Si chips placed in the wells of a microtiter plate. The assay platform showed a 100 fg·mL−1 limit of detection for both PSA and hK2, and a dynamic range that extends over five orders of magnitude. After optimization of the density of both capture antibodies, neither the PSA nor the hK2 array showed cross-sensitivity to non-target proteins or other plasma proteins. The microarray was evaluated by titration of PSA and hK2, respectively, in the same serum samples. In our perception, this highly sensitive and selective platform holds promise for improved detection of tumor markers in an early diagnostic stage, but also to monitor the recurrence of prostate cancer. [Figure not available: see fulltext.]


External organisations
  • University of Tokyo
  • Dongguk University, Seoul
  • Memorial Sloan-Kettering Cancer Center
  • University of Oxford
  • University of Tampere
  • RIKEN Bioengineering Laboratory
  • Inje University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry


  • Duplex antibody microarray, hK2, Prostate cancer, PSA, Serum analysis, Silicon anodization
Original languageEnglish
Pages (from-to)3321-3327
Number of pages7
JournalMikrochimica Acta
Issue number12
Early online date2016 Oct 19
Publication statusPublished - 2016 Dec
Publication categoryResearch