Predictability of heterozygosity scores and polymorphism information content values for rat genetic markers

Research output: Contribution to journalArticle

Abstract

Construction of a genetic linkage map of the laboratory rat, Rattus norvegicus, establishes the rat as a genetic model. Allele sizes were reported for 432 simple sequence length polymorphisms (SSLPs) genotyped in 12 different substrains belonging to nine different inbred strains of rats. However, these nine strains represent only a fraction of the more than 140 inbred strains available. If allele sizes are not known, alternative indices of markers' polymorphism content can be used, such as heterozygosity (H) and polymorphism information content (PIC). Here, we have determined heterozygosity scores and PIC values for all markers of the rat genetic linkage map, and we evaluate the predictability of the heterozygosity and the PIC values. Correlation analysis between the nine inbred strains reported for the rat map and ten "test" strains yielded r=0.42 and r=0.44 for heterozygosity and PIC values, respectively. While the correlation of the indices between the two groups of animals is low, these indices do provide a means of predicting whether a genetic marker will be informative in strains where allele sizes are not known.

Details

Authors
  • A. Pettersson
  • E. S. Winer
  • S. Weksler-Zangen
  • Å Lernmark
  • H. J. Jacob
External organisations
  • Massachusetts General Hospital
  • Karolinska University Hospital
Original languageEnglish
Pages (from-to)512-520
JournalMammalian Genome
Volume6
Issue number8
Publication statusPublished - 1995 Aug 1
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes