Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

Research output: Contribution to journalArticle

Abstract

Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.

Details

Authors
External organisations
  • University of Gothenburg
Research areas and keywords

Keywords

  • Albumins, Alzheimer Disease, Cerebrospinal Fluid, Chromatography, Affinity, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Glycopeptides, Glycoproteins, Humans, Hydrogen-Ion Concentration, Isoelectric Focusing, Mass Spectrometry, Polysaccharides, Protein Isoforms, Spectroscopy, Fourier Transform Infrared, Trypsin, Journal Article, Research Support, Non-U.S. Gov't
Original languageEnglish
Pages (from-to)2294-301
Number of pages8
JournalJournal of Proteome Research
Volume4
Issue number6
Publication statusPublished - 2005 Dec 13
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes