Preparation of protein imprinted polymer beads by Pickering emulsion polymerization

Research output: Contribution to journalArticle

Standard

Preparation of protein imprinted polymer beads by Pickering emulsion polymerization. / Zhou, Tongchang; Zhang, Ka; Kamra, Tripta; Bülow, Leif; Ye, Lei.

In: Journal of Materials Chemistry. B, Vol. 3, No. 7, 2015, p. 1254-1260.

Research output: Contribution to journalArticle

Harvard

APA

CBE

MLA

Vancouver

Author

RIS

TY - JOUR

T1 - Preparation of protein imprinted polymer beads by Pickering emulsion polymerization

AU - Zhou, Tongchang

AU - Zhang, Ka

AU - Kamra, Tripta

AU - Bülow, Leif

AU - Ye, Lei

PY - 2015

Y1 - 2015

N2 - We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.

AB - We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.

U2 - 10.1039/c4tb01605j

DO - 10.1039/c4tb01605j

M3 - Article

VL - 3

SP - 1254

EP - 1260

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-7518

IS - 7

ER -