Prevalence of G6PD deficiency and associated haematological parameters in children from Botswana

Research output: Contribution to journalArticle

Abstract

Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is commonly seen in malaria endemic areas as it is known to confer a selective advantage against malaria. Recently, we reported a high proportion of asymptomatic reservoir of Plasmodium vivax in Botswana, that calls for intervention with primaquine to achieve radical cure of vivax malaria. Considering that individuals with this enzyme deficiency are at risk of haemolysis following primaquine treatment, assessment of the population for the relative frequency of G6PD deficiency is imperative. Samples from 3019 children from all the districts of Botswana were successfully genotyped for polymorphisms at positions 202 and 376 of the G6PD gene. Haematological parameters were also measured. The overall population allele frequency (based on the hemizygous male frequency) was 2.30% (95% CI, 1.77–2.83), while the overall frequency of G6PD-deficient genotypes A- (hemizygote and homozygote genotypes only) was 1.26% (95% CI, 0.86–1.66). G6PD deficiency is spread in Botswana according to the historical prevalence of malaria with a North-West to South-East decreasing gradient trend. There was no association between G6PD status and P. vivax infection. G6PD A- form was found to be associated with decreased RBC count and haemoglobin levels without a known cause or illness. In conclusion, we report for the first time the prevalence of G6PD deficiency in Botswana which is relevant for strategies in the malaria elimination campaign. Further work to examine the activities of the enzyme in the Botswana population at risk for malaria is warranted.

Details

Authors
  • Thato Motshoge
  • Grace Ababio
  • Larysa Aleksenko
  • Sajini Souda
  • Charles Waithaka Muthoga
  • Naledi Mutukwa
  • Leabaneng Tawe
  • Pleasure Ramatlho
  • Lesego Gabaitiri
  • Simon Chihanga
  • Tjantilili Mosweunyane
  • Shimeles Hamda
  • Kentse Moakofhi
  • Davies Ntebela
  • Elias Peloewetse
  • Loeto Mazhani
  • Jeffrey M. Pernica
  • John Read
  • Isaac Kweku Quaye
  • Giacomo Maria Paganotti
Organisations
External organisations
  • University of Botswana
  • University of Ghana
  • University of Pennsylvania
  • Ministry of Health and Wellness, Botswana
  • University of Namibia
  • Botswana-University of Pennsylvania Partnership
  • Sub-Saharan African Network for TB/HIV Research Excellence (SANTHE)
  • World Health Organization (WHO)
  • McMaster University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Public Health, Global Health, Social Medicine and Epidemiology

Keywords

  • Botswana, G6PD deficiency, Malaria, Plasmodiun vivax, Primaquine
Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalInfection, Genetics and Evolution
Volume63
Publication statusPublished - 2018 Sep 1
Publication categoryResearch
Peer-reviewedYes