Processes affecting genetic structure and conservation: a case study of wild and cultivated Brassica rapa

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Processes affecting genetic structure and conservation: a case study of wild and cultivated Brassica rapa. / Andersen, Naja Steen; Poulsen, Gert; Andersen, Bente Anni; Kiaer, Lars Podenphant; D'Hertefeldt, Tina; Wilkinson, Mike J.; Jorgensen, Rikke Bagger.

In: Genetic Resources and Crop Evolution, Vol. 56, No. 2, 2009, p. 189-200.

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Andersen, Naja Steen ; Poulsen, Gert ; Andersen, Bente Anni ; Kiaer, Lars Podenphant ; D'Hertefeldt, Tina ; Wilkinson, Mike J. ; Jorgensen, Rikke Bagger. / Processes affecting genetic structure and conservation: a case study of wild and cultivated Brassica rapa. In: Genetic Resources and Crop Evolution. 2009 ; Vol. 56, No. 2. pp. 189-200.

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TY - JOUR

T1 - Processes affecting genetic structure and conservation: a case study of wild and cultivated Brassica rapa

AU - Andersen, Naja Steen

AU - Poulsen, Gert

AU - Andersen, Bente Anni

AU - Kiaer, Lars Podenphant

AU - D'Hertefeldt, Tina

AU - Wilkinson, Mike J.

AU - Jorgensen, Rikke Bagger

PY - 2009

Y1 - 2009

N2 - When planning optimal conservation strategies for wild and cultivated types of a plant species, a number of influencing biological and environmental factors should be considered from the outset. In the present study Brassica rapa was used to illustrate this: to develop Scandinavian conservation strategies for wild and cultivated B. rapa, DNA-marker analysis was performed on 15 cultivated and 17 wild accessions of B. rapa plus 8 accessions of the cross compatible B. napus. The B. rapa cultivars were bred in Sweden and Finland in 1944-1997 and the wild B. rapa material was collected from Denmark, Sweden and United Kingdom. The B. napus accessions were bred within the last 20 years in the Scandinavian countries. Results were based on scoring of 131 polymorphic ISSR markers in the total plant material. A Bayesian Markov chain Monte Carlo (MCMC) approach implemented in NewHybrids demonstrated a clear distinction of B. rapa and B. napus individuals except for three individuals that seemed to be backcrosses. The backcrossed hybrids descended from two Swedish populations, one wild and one escaped. The overall pattern of genetic variation and structure in B. rapa showed that cultivated and wild B. rapa accessions formed two almost separated clusters. Geographical origin and breeding history of cultivars were reflected in these genetic relationships. In addition, wild populations from Denmark and Sweden seemed to be closely related, except for a Swedish population, which seemingly was an escaped cultivar. The study point to that many processes, e.g. spontaneous introgression, naturalisation, breeding and agricultural practise affected the genetic structure of wild and cultivated B. rapa populations.

AB - When planning optimal conservation strategies for wild and cultivated types of a plant species, a number of influencing biological and environmental factors should be considered from the outset. In the present study Brassica rapa was used to illustrate this: to develop Scandinavian conservation strategies for wild and cultivated B. rapa, DNA-marker analysis was performed on 15 cultivated and 17 wild accessions of B. rapa plus 8 accessions of the cross compatible B. napus. The B. rapa cultivars were bred in Sweden and Finland in 1944-1997 and the wild B. rapa material was collected from Denmark, Sweden and United Kingdom. The B. napus accessions were bred within the last 20 years in the Scandinavian countries. Results were based on scoring of 131 polymorphic ISSR markers in the total plant material. A Bayesian Markov chain Monte Carlo (MCMC) approach implemented in NewHybrids demonstrated a clear distinction of B. rapa and B. napus individuals except for three individuals that seemed to be backcrosses. The backcrossed hybrids descended from two Swedish populations, one wild and one escaped. The overall pattern of genetic variation and structure in B. rapa showed that cultivated and wild B. rapa accessions formed two almost separated clusters. Geographical origin and breeding history of cultivars were reflected in these genetic relationships. In addition, wild populations from Denmark and Sweden seemed to be closely related, except for a Swedish population, which seemingly was an escaped cultivar. The study point to that many processes, e.g. spontaneous introgression, naturalisation, breeding and agricultural practise affected the genetic structure of wild and cultivated B. rapa populations.

KW - Crop-weed complex

KW - Conservation

KW - Brassica napus

KW - Brassica rapa

KW - Genetic

KW - differentiation

KW - ISSR

U2 - 10.1007/s10722-008-9354-6

DO - 10.1007/s10722-008-9354-6

M3 - Article

VL - 56

SP - 189

EP - 200

JO - Genetic Resources and Crop Evolution

T2 - Genetic Resources and Crop Evolution

JF - Genetic Resources and Crop Evolution

SN - 0925-9864

IS - 2

ER -