Progenitor cell-derived factors enhance photoreceptor survival in rat retinal explants.

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Progenitor cell-derived factors enhance photoreceptor survival in rat retinal explants. / Liljekvist Soltic, Ingela; Olofsson, Jenny; Johansson, Kjell.

In: Brain Research, Vol. 1227, 2008, p. 226-233.

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Liljekvist Soltic, Ingela ; Olofsson, Jenny ; Johansson, Kjell. / Progenitor cell-derived factors enhance photoreceptor survival in rat retinal explants. In: Brain Research. 2008 ; Vol. 1227. pp. 226-233.

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TY - JOUR

T1 - Progenitor cell-derived factors enhance photoreceptor survival in rat retinal explants.

AU - Liljekvist Soltic, Ingela

AU - Olofsson, Jenny

AU - Johansson, Kjell

PY - 2008

Y1 - 2008

N2 - Explantation of postnatal rat retinas is associated with degenerative events that show morphological similarities to human retinal degenerative disorders. The most evident morphological features are photoreceptor apoptosis involving caspase-3 and Müller cell activation. The purpose of the present study was to determine the content of protective factors in rat retinal progenitor cells and analyze the influence of the identified factors on the survival of photoreceptor cells and retinal gliosis. Tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) and vascular endothelial growth factor (VEGF) were identified as putative beneficial factors, and their combined effect was examined in rat retinal explant cultures. Photoreceptor apoptosis was estimated by cell counts of cleaved caspase-3 and caspase-12 immunolabeled as well as TUNEL labeled cells. TIMP-1 and VEGF in combination significantly suppressed photoreceptor apoptosis involving caspase-3 activation. Cell counts of caspase-12 and TUNEL labeled photoreceptors showed no significant difference between the experiment and control retinas. TIMP-1 and VEGF appeared to have no effect on Müller cell activation as measured by GFAP and Ki-67 immunohistochemistry. Our data suggest that TIMP-1 and VEGF in combination promote the survival of photoreceptor cells in rat retinal explants, possibly by affecting a caspase-3 signaling pathway.

AB - Explantation of postnatal rat retinas is associated with degenerative events that show morphological similarities to human retinal degenerative disorders. The most evident morphological features are photoreceptor apoptosis involving caspase-3 and Müller cell activation. The purpose of the present study was to determine the content of protective factors in rat retinal progenitor cells and analyze the influence of the identified factors on the survival of photoreceptor cells and retinal gliosis. Tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) and vascular endothelial growth factor (VEGF) were identified as putative beneficial factors, and their combined effect was examined in rat retinal explant cultures. Photoreceptor apoptosis was estimated by cell counts of cleaved caspase-3 and caspase-12 immunolabeled as well as TUNEL labeled cells. TIMP-1 and VEGF in combination significantly suppressed photoreceptor apoptosis involving caspase-3 activation. Cell counts of caspase-12 and TUNEL labeled photoreceptors showed no significant difference between the experiment and control retinas. TIMP-1 and VEGF appeared to have no effect on Müller cell activation as measured by GFAP and Ki-67 immunohistochemistry. Our data suggest that TIMP-1 and VEGF in combination promote the survival of photoreceptor cells in rat retinal explants, possibly by affecting a caspase-3 signaling pathway.

U2 - 10.1016/j.brainres.2008.06.077

DO - 10.1016/j.brainres.2008.06.077

M3 - Article

VL - 1227

SP - 226

EP - 233

JO - Brain Research

JF - Brain Research

SN - 1872-6240

ER -