Progenitor cells in the postnatal central nervous system, characterization and genetic manipulation
Research output: Thesis › Doctoral Thesis (compilation)
New neurons are only generated in two places of the postnatal brain; the subventricular zone (SVZ) and hippocampus. The SVZ-derived cells specifically migrate to the olfactory bulb where they give rise to new interneurons. We have genetically manipulated the postnatal SVZ progenitor cells with the aim to direct them to a neuronal fate in the striatum. Our result showed that ectopic expression of the transcription factors Islet1 and Neurogenin2 directed the SVZ cells progeny to a new fate in the striatum and external capsule. Once removed to the culture dish they expressed the neuronal marker ?-III-tubulin. Thus, showing that ectopic expression of Islet1 and Neurogenin2 in the postnatal SVZ progenitor cells promote striatal recruitment for their progeny, which have potential to differentiate into neurons.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Award date||2007 Jun 9|
|Publication status||Published - 2007|
Defence details Date: 2007-06-09 Time: 09:00 Place: Segerfalksalen, Wallenberg Neurocentrum, Lund. External reviewer(s) Name: Eriksson, Peter Title: Professor Affiliation: Sektionen för klinisk neurovetenskap och rehabilitering ---
Nina Rogelius, Cecilia Ericson and Cecilia Lundberg. 2005. In vivo labeling of neuroblasts in the subventricular zone of rats. J Neurosci Methods, vol 142 pp 285-293.
Nina Rogelius, B Josephine Jensen, Cecilia Lundberg and Malin Parmar. 2006. Retrovirally delivered Islet-1 increases recruitment of ng2 expressing cells from the postnatal SVZ into the striatum. Experimental Neurology, vol 201 pp 388-398.
Nina Rogelius, B Josephine Jensen, Cecilia Lundberg and Malin Parmar. . Expression of Isl1 and Ngn2 in neonatal SVZ results in a striatal cell populatioin with potential for neuronal differentiation. (submitted)
No data available