Prognostic Impact of Tumor Cell Programmed Death Ligand 1 Expression and Immune Cell Infiltration in NSCLC

Research output: Contribution to journalArticle

Abstract

Introduction: Infiltration of T and B/plasma cells has been linked to NSCLC prognosis, but this has not been thoroughly investigated in relation to the expression of programmed death ligand 1 (PD-L1). Here, we determine the association of lymphocytes and PD-L1 with overall survival (OS) in two retrospective cohorts of operated NSCLC patients who were not treated with checkpoint inhibitors targeting the programmed death 1/PD-L1 axis. Moreover, we evaluate how PD-L1 positivity and clinicopathologic factors affect the prognostic association of lymphocytes. Methods: Cluster of differentiation (CD) 3 (CD3)-, CD8-, CD4-, forkhead box P3 (FOXP3)-, CD20-, CD79A-, and immunoglobulin kappa constant (IGKC)-positive immune cells, and tumor PD-L1 positivity, were determined by immunohistochemistry on tissue microarrays (n = 705). Affymetrix data was analyzed for a patient subset, and supplemented with publicly available transcriptomics data (N = 1724). Associations with OS were assessed by Kaplan-Meier plots and uni- and multivariate Cox regression. Results: Higher levels of T and B plasma cells were associated with longer OS (p = 0.004 and p < 0.001, for CD8 and IGKC, respectively). Highly proliferative tumors with few lymphocytes had the worst outcome. No association of PD-L1 positivity with OS was observed in a nonstratified patient population; however, a significant association with shorter OS was observed in never-smokers (p = 0.009 and p = 0.002, 5% and 50% cutoff). Lymphocyte infiltration was not associated with OS in PD-L1–positive tumors (50% cutoff). The prognostic association of lymphocyte infiltration also depended on the patients’ smoking history and histologic subtype. Conclusions: Proliferation, PD-L1 status, smoking history, and histology should be considered if lymphocyte infiltration is to be used as a prognostic biomarker.

Details

Authors
  • Karolina Edlund
  • Katrin Madjar
  • Johanna S.M. Mattsson
  • Dijana Djureinovic
  • Cecilia Lindskog
  • Hans Brunnström
  • Hirsh Koyi
  • Eva Brandén
  • Karin Jirström
  • Fredrik Pontén
  • Jörg Rahnenführer
  • Patrick Micke
  • Jan G. Hengstler
Organisations
External organisations
  • Leibniz Research Centre for Working Environment and Human Factors (IfADo)
  • Uppsala University
  • Gävle Hospital
  • Technical University of Dortmund
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology

Keywords

  • Adenocarcinoma, Ki67, Lymphocyte, Prognosis, Squamous cell carcinoma
Original languageEnglish
JournalJournal of Thoracic Oncology
Publication statusE-pub ahead of print - 2019 Feb 9
Publication categoryResearch
Peer-reviewedYes