Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis.

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Abstract

Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.

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Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology
  • Microbiology

Keywords

  • CtaA, Heme A synthesis, Membrane protein evolution, Cytochrome a
Original languageEnglish
Pages (from-to)1330-1334
JournalFEBS Letters
Volume582
Publication statusPublished - 2008
Publication categoryResearch
Peer-reviewedYes