Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis.

Research output: Contribution to journalArticle


Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology
  • Microbiology


  • CtaA, Heme A synthesis, Membrane protein evolution, Cytochrome a
Original languageEnglish
Pages (from-to)1330-1334
JournalFEBS Letters
Publication statusPublished - 2008
Publication categoryResearch