Protein separation using metal ion-bound particles in aqueous two-phase system

Research output: Contribution to journalArticle

Abstract

Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.

Details

Authors
Organisations
External organisations
  • Kyonggi University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Bioprocess Technology

Keywords

  • Affinity partitioning, Aqueous two-phase system, Lysozyme, Protein separation
Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalBiotechnology Techniques
Volume13
Issue number2
Publication statusPublished - 1999 Apr 14
Publication categoryResearch
Peer-reviewedYes