Proteomic Discovery and Development of a Multiplexed Targeted MRM-LC-MS/MS Assay for Urine Biomarkers of Extracellular Matrix Disruption in Mucopolysaccharidoses I, II, and VI

Research output: Contribution to journalArticle

Abstract

The mucopolysaccharidoses (MPS) are lysosomal storage disorders that result from defects in the catabolism of glycosaminoglycans. Impaired muscle, bone, and connective tissue are typical clinical features of MPS due to disruption of the extracellular matrix. Markers of MPS disease pathology are needed to determine disease severity and monitor effects of existing and emerging new treatments on disease mechanisms. Urine samples from a small cohort of MPS-I, -II, and -VI patients (n = 12) were analyzed using label-free quantative proteomics. Fifty-three proteins including many associated with extracellular matrix organization were differently expressed. A targeted multiplexed peptide MRM LC-MS/MS assay was used on a larger validation cohort of patient samples (MPS-I n = 18, MPS-II n = 12, MPS-VI n = 6, control n = 20). MPS-I and -II groups were further subdivided according to disease severity. None of the markers assessed were altered significantly in the mild disease groups compared to controls. β-galactosidase, a lysosomal protein, was elevated 3.6-5.7-fold significantly (p < 0.05) in all disease groups apart from mild MPS-I and -II. Collagen type Iα, fatty-acid-binding-protein 5, nidogen-1, cartilage oligomeric matrix protein, and insulin-like growth factor binding protein 7 concentrations were elevated in severe MPS I and II groups. Cartilage oligomeric matrix protein, insulin-like growth factor binding protein 7, and β-galactosidase were able to distinguish the severe neurological form of MPS-II from the milder non-neurological form. Protein Heg1 was significantly raised only in MPS-VI. This work describes the discovery of new biomarkers of MPS that represent disease pathology and allows the stratification of MPS-II patients according to disease severity.

Details

Authors
  • Wendy E. Heywood
  • Stephane Camuzeaux
  • Ivan Doykov
  • Nina Patel
  • Rhian Lauren Preece
  • Emma Footitt
  • Maureen Cleary
  • Peter Clayton
  • Stephanie Grunewald
  • Lara Abulhoul
  • Anupam Chakrapani
  • Neil J. Sebire
  • Peter Hindmarsh
  • Tom J. De Koning
  • Simon Heales
  • Derek Burke
  • Paul Gissen
  • Kevin Mills
External organisations
  • UCL Institute of Child Health
  • Royal Free Hospital
  • University Medical Center Groningen
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Clinical Laboratory Medicine
Original languageEnglish
Pages (from-to)12238-12244
Number of pages7
JournalAnalytical Chemistry
Volume87
Issue number24
Publication statusPublished - 2015 Dec 15
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes