Protocol for Proteome Analysis of Group A Streptococcus

Research output: Chapter in Book/Report/Conference proceedingBook chapter

Abstract

This chapter describes a protocol for the separation of different cell fractions from Streptococcus pyogenes and sample preparation for analysis by mass spectrometry. The presented approach can be used for the analysis of all subcellular proteomes from S. pyogenes and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.

Details

Authors
  • Laura Wilk
Organisations
External organisations
  • Robert Koch Institut
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry
  • Pharmaceutical Sciences

Keywords

  • Bacterial proteome, Cell wall, Cellular fractionation, Gram-positive bacteria, Mass spectrometry, Membrane, secretome, Streptococcus pyogenes
Original languageEnglish
Title of host publicationGroup A Streptococcus
Subtitle of host publicationMethods and Protocols
EditorsThomas Proft , Jacelyn M.S. Loh
PublisherHumana Press
Chapter9
Pages135-144
Number of pages10
ISBN (Electronic)978-1-0716-0467-0
ISBN (Print)978-1-0716-0466-3
Publication statusPublished - 2020 May 20
Publication categoryResearch
Peer-reviewedYes

Publication series

NameMethods in Molecular Biology
Volume2136
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029